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155 The multi-nutrient tablet provided: In an uncontrolled clinical trial that included 31 participants with one-sided tinnitus, 18 weeks of treatment with a multi-nutrient supplement, providing glycerophosphorylcholine (55 mg/day), glycerophosphorylethanolamine (45 mg/day), beta-carotene (6,000 mcg RAE/day), vitamin C (180 mg/day), and vitamin E (10 mg/day), led to reductions in tinnitus loudness and discomfort
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Explants were then washed in PBS and immunostained using Chicken Neurofilament (marker for SGNs), Mouse Myosin VIIa (Hair cell marker), Phalloidin (actin marker) and DAPI (nuclear staining)