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FcRn binding to HA-Fc fusions in ELISA 96-well EIA/RIA plates (Corning) were coated with a recombinant human albumin variant (8 μg/mL in PBS/ 100 μL per well) engineered to bind pH independently to human FcRn 29

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This “post-surgical gut paralysis” renders some patients unable to eat — while saddling them with serious constipation, bloating, and discomfort and keeping them in hospital much longer than they otherwise would have been