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The GFP signals were detected with the FITC channel, using a detector gain that positions the negative control cell peak around 1 × 10 2 and 1 × 10 3

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they frequently lack appropriate specificity, chemical stability, or interference resistance to cellular components [115] and they are mostly incapable of simultaneously quantifying the intricate network of interconnected redox couples (e.g., GSH/GSSG, NAD⁺/NADH, and Trx redox states) with adequate sensitivity under physiologically relevant conditions [116]